In an alternative preparation of the hydrocarbylsulfonic acid, the hydrocarbon is first reacted with an alkyl chlorosulfonate, optionally in the presence of a solvent such as 1,2-dichloroethane, ether, and the like. The ring grooves are rated on a scale of 0 to 100 groove fill, with 0 representing clean. Analysis: Cl, 0.024%w; S, 1.06%w; N, 0.57%w. Solvent For the most part, reactive dyes and haptens are hydrophobic molecules and should be dissolved in high-quality, anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO). Ordinarily, the reaction takes place in about 1 to about 24 hours, and usually in about 2 to about 8 hours. In particular, when using tetramethylrhodamine (TAMRA), redissolve the oligonucleotide pellet in deionized water, and repeat the ethanol precipitation. 3,172,892, 3,219,666, 3,381,022; neutral and basic calcium, barium and magnesium petrosulfonates or alkyl phenates; oxidation inhibitors, antifoam agents, viscosity index improvers, pour-point depressants, and the like, such as chlorinated wax, benzyldisulfide, sulfurized sperm oil, sulfurized terpene; phosphorus esters such as trihydrocarbon phosphites and phosphates; metal thiocarbamates such as zinc dioctyldithiocarbamate; metal phosphorus dithioates such as zinc dioctylphosphorodithioate; polyisobutene having an average molecular weight of 100,000; etc. Particularly preferred are substantially equal molar amounts of acid and polymer (on the basis of one double bond per polymer molecule). Draw structures of SF4 in a way that indicates their molecular shape? Thus, for reasons of oil solubility and stability, the interpolymers contemplated for use in this invention should be substantially aliphatic and substantially saturated, i.e., they should contain at least about 80%, preferably about 95%, on a weight basis, of units derived from the aliphatic monoolefins and no more than about 5% of olefin linkages based on the total number of carbon-to-carbon covalent linkages. NHS ester reaction scheme for chemical conjugation to a primary amine. 409.9 g of chlorophenyl polyisobutenylethylsulfonate (1.62%w sulfur) and 280.1 g (10.5 equivalent ratio) of n-octylamine are reacted at 160° C. for 3 hours. The carbyl sulfate and the aromatic hydroxy compound usually react in equivalent quantities, i.e., a molar ratio of about 1:1 for monochydric phenols and about a 1:2 phenol/carbyl sulfate ratio for dihydric phenols. A fully satisfactory technique is to charge the hydrocarbyl substituent source to the reaction vessel and heat with stirring. Preferred compounds contain a total of 25 to 35 carbon atoms between R and R1. The other branch of the Y tube is outfitted with a pressure-compensated dropping funnel. In a sixth embodiment, lubricating oil compositions comprise: (a) a major amount of an oil of lubricating viscosity, and (b) an amount effective to provide dispersancy of an oil-soluble, nitrogen-containing composition prepared by: (1) reacting an aryl ester of a substantially saturated hydrocarbylethylsulfonic acid or (2) a substantially saturated hydrocarbylsulfonyl chloride with a nitrogen-containing compound containing at least one amine hydrogen, i.e., --NH function, in which the substantially saturated hydrocarbyl subtituent contains at least 20 aliphatic carbon atoms. The solvent is removed by stripping at 190° C. at 5 mm Eg to yield 475 g of product. A vacuum is applied to the kettle and the contents are heated to 165° C. maximum to distill off the thinner and methanol. The mixture is allowed to stand at ambient temperature for 13/4 hours. PURIFYING THE AMINE-MODIFIED OLIGONUCLEOTIDE To ensure that the oligonucleotide is free of interfering compounds, especially amines, such as triethylamine or Tris, and ammonium salts, we strongly recommend extracting and precipitating the sample prior to initiating the labeling reaction. I. Benzyl Esters from Methyl Esters and Benzyldimethylamine. Reactivity and Molecular Recognition: Amine Methylation by an Introverted Ester. V.S. 1100 ml) is decanted. Polymers of olefins in which the olefinic linkage is not at the terminal position likewise are useful. An additional 200 ml of methanol and 200 ml of hydrocarbon thinner are added and the mixture is stirred for 2 minutes. The protein concentration in the reaction should usually be 5–20 mg/mL. Analysis: Cl, 0.96%w; S, 0.97%w; N, 0.47%w. Determine the amount of steam (in g) needed for the system to reach a final temperature of 48.0°C. Günther Drefahl, Hans Lückert, Wolfgang Köhler. First react a grignard reagent with amine to get RNHMgBr and then add your ester to the reaction mixture. The reaction between ethylene and sulfur trioxide rapidly takes place with evolution of heat of reaction to yield carbyl sulfate. The reaction product is stripped under vacuum and sparged with nitrogen to yield 24.0 g. The product is diluted with 8.0 g of a 100 SUS neutral solvent-refined lubricating oil. These compounds, prepared by reacting an olefin polymer with chlorosulfonic acid and then with an amine, are useful as detergents in lubricating oil compositions. These compositions have excellent detergency and dispersancy properties in lubricating oils. This article is cited by Suitable lubricating oils which can be used as diluents typically have viscosities in the range from about 35 to about 1000 Saybolt Universal Seconds (SUS) at 100° F., although any oil of lubricating viscosity can be used. The reaction releases N-hydroxysuccinimide (NHS). This is readily determined by comparing the bubbling rate in the bubbler attached to the sulfur trioxide addition funnel and the bubbler attached to the outlet of the condenser on the 5-liter flask. For optimal results, we recommend using a tetraborate buffer at pH 8.5, rather than the bicarbonate buffers recommended for protein conjugations. The amount of excess chlorosulfonic acid employed may vary widely, however, and will be a function of the amount of diluent employed as well as the desired rate of reaction. Note: Treatment with hydroxylamine at this stage is required to remove the trifluoroacetyl protecting groups of Rhodamine Green™ (Cat. 2105 g of the sulfonate used in Example 4I and 309 g (3 mol ratio) of diethylenetriamine are reacted at 160° C. for 6 hours. The mixture is allowed to stand at ambient temperature for about 31/2 hours. Completely redissolve this material by extensive vortex mixing before centrifugation. The sulfonate was previously dried by using a benzene azeotrope and the amine was dried over calcium hydride. In the first procedure, the hydrocarbon is reacted with a sulfonating agent, preferably chlorosulfonic acid. The reaction mass is diluted wtih 0.5% vol. The relative proportions of the monoolefins to the other monomers in the interpolymers influence the stability and oil solubility of the final compositions derived from such interpolymers. No. 600 ml of n-hexane is added to the flask and the mixture is stirred for 2 minutes. Dietrich, U.S. Pat. The increase is reported as a percentage of the original value. The reaction mass is stripped under vacuum and sparged with nitrogen for 5 hours to yield 1522 g of product. The kinetics and mechanism of the N,N,N′,N′-tetramethylxylylenediamine polycondensation with dicarboxylates in the melt.
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